25th EAA Annual Meeting in Bern
[Acceptance Letter by EAA]
Dear Jong Ha
Let me congratulate you on the acceptance of your contribution at the 25th EAA Annual Meeting in Bern.
Contribution type: Oral presentation
Contribution ID: 1686
Contribution title: Ancient mitochondrial DNA analysis of Ring-necked Pheasant (Phasianus colchicus) bones collected from 4th century archaeological site in South Korea
Accepted into session no. 280 "New approaches in bioarchaeology"
The list of accepted contributions for EAA meeting
Ancient mitochondrial DNA analysis of Ring-necked Pheasant (Phasianus colchicus) bones collected from 4th century archaeological site in South Korea
Jong Ha Honga, Chang Seok Oha, Hwan-il Kimb, Eun Jin Wooc, Tae sop Chod, Dong Hoon Shina,＊
a Lab of Bioanthropology Paleopathology and History of Disease, Department of Anatomy and Cell Biology / Institute of Forensic Science, Seoul National University College of Medicine, Seoul, South Korea
b Central Institute of Cultural Heritage
c Department of History, Sejong University, Seoul, South Korea
d Department of History, Yonsei University, Seoul, South Korea
Dong Hoon Shin, MD, PhD, MS.
Lab of Bioanthropology, Paleopathology and History of Diseases
Institute of Forensic Medicine, Seoul National University College of Medicine,
103, Daehak-ro, Jongno-gu, Seoul 03080, South Korea
E-mail: email@example.com; Fax: +82 2 745 9528
E-mail addresses: firstname.lastname@example.org (D.H. Shin).
Ancient DNA (aDNA) analysis becomes widely used nowadays to get genetic information from the ancient avian bones discovered at archaeological sites. However, as for Ring-necked Pheasants (Phasianus colchicus), though it was closely related to human culture and society for a long time, detailed genetic history could not be inferred from aDNA analysis. In this study, we thus carried out the first research on the ancient P. colchicus DNA recovered from 4th century avian bones found in South Korea. We used femurs of two avian individuals (K-14 and K-15) for amplification, cloning and sequencing of P. colchicus aDNA. In NCBI/BLAST search, the haplotypes of the current ancient pheasants (K-14 and K-15) were similar to modern P. colchicus DNAs (P. c. pallasi, P. c. strauchi, P. c. elegans, P. c. torqualus, P. c. kengsuensis and P. c. karpowi etc.) that belong to the Eastern clade of Ring-necked Pheasant subspecies. The ancient pheasant DNAs (K-14 and K-15) obtained in the current study were the genetically closest to P.c. pallasi, the subspecies commonly found in Manchuria and Korean peninsula. Through this analysis, we managed to reveal fundamental genetic traits of the ancient Ring-necked Pheasants existed long ago in East Asia, thus providing fundamental phylogeographical information of P. colchicus DNA across time and space.
Keywords: Ring-necked Pheasant; ancient DNA; phylogenetic analysis; ancient bone