[Presentation] The 3rd Annual Conference of the Japanese Society of Paleopathology
DNA Sequences of 28S rRNA and COI regions obtained from ancient Metagonimus yokogawai eggs found in the coprolites of Joseon Dynasty mummies
Jong Ha Hong1, Chang Seok Oh1,2, Myeung Ju Kim3, Min Seo4,*, Dong Hoon Shin1,2,*
1Laboratory of Bioanthropology, Paleopathology and History of Diseases, Seoul National University College of Medicine, Seoul, South Korea; 2Institute of Forensic Science, Seoul National University College of Medicine, Seoul, South Korea; 3Department of Anatomy, Dankook University College of Medicine, Cheonan, South Korea; 4Department of Parasitology, Dankook University College of Medicine, Cheonan, South Korea; *Co-correspondences.
In this report, we present our research on Metagonimus yokogawai DNA sequences obtained from ancient parasite eggs remained in coprolite specimens of the 15th to 18th century Joseon mummies. We successfully amplified and sequenced ancient Metagonimus 28S rRNA and COI DNA regions (n=2). The consensus sequences were then determined by the alignment of the cloned sequences of PCR products. In BLAST and phylogenetic analyses, we found that the current M. yokogawai sequences of Joseon mummies were clustered together with M. miyatai, M. takahashi, and M. pusillus; however, they were evidently differentiated from species other than genus Metagonimus spp. We also discovered that each M. yokogawai taxon was not clustering by regional differences of the sampling. This study is the first of its kind to identify ancient Metagonimus DNA from archaeological specimens. Our research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2017R1D1A1B03030127).