Dong Hoon Shin MD, PhD, MS. Lab of Bioanthropology, Paleopathology and History of Diseases, Department of Anatomy/Institute of Forensic Science, Seoul National University College of Medicine, 103 Daehak-ro (Yongon-dong), Jongno-gu, Seoul 03080, South Korea. E-mail: firstname.lastname@example.org(DH Shin); TEL: +82-2-740-8203
[Presentation] 24th Annual Meeting of European Association of Archaeologists-.
DNA Sequences of CO1 and ITS1 genes obtained from ancient Clonorchis sinensis eggs remained in Joseon Dynasty mummies
Jong Ha Hong1, Chang Seok Oh1,2, Min Seo3,*, Dong Hoon Shin1,2,*
1Laboratory of Bioanthropology, Paleopathology and History of Diseases, Seoul National University College of Medicine, Seoul, South Korea; 2Institute of Forensic Science, Seoul National University College of Medicine, Seoul, South Korea; 3Department of Parasitology, Dankook University College of Medicine, Cheonan, South Korea
The parasitological information obtained from archaeological specimens can propose speculation about the parasite-infection patterns that prevailed in ancient societies. In this study, we thus analyzed Clonorchis sinensis (C. sinensis) DNA extracted from the ancient feces or liver of 16th-18th century Korean mummies, especially on Cytochrome C Oxidase Subunit 1 (CO1) and internal transcribed spacer 1 (ITS1) genes. The mummies (n=5; Mungyeong, Dalsung, Cheongdo, Hadong1 and Andong) found in the graves of Joseon period (1392-1910 CE) were used in this study. This kind of graves was used mainly by the upper-class people of Joseon period. The samples are ancient feces obtained from mummy intestine (Dalsung, Hadong1 and Andong); or the liver (Mungyeong and Cheongdo). After C. sinensis genes were successfully amplified from two mummies (Mungyeong and Andong), consensus sequences were determined by the alignment of the sequences of cloned PCR products. The obtained CO1 and ITS1 sequences were well matched with those of already reported C. sinensis, but can be clearly differentiated from other species such as Opisthorchis viverrini and Pseudamphistomum truncatum, etc. By the phylogenetic analysis of the ITS1 gene, we tried to see the clustering according to regional differences of the C. sinensis examined. This study shows the genetic characteristics of ancient C. sinensis in Joseon period of Korea by comparison with those of modern C. sinensis sequences worldwide. To improve the knowledge about C. sinensis evolution in much detail, C. sinensis gene sequences must be obtained from the regions of much wider geo-historical scope in forthcoming studies. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (no. NRF-2016R1A2B4015669). For any queries about this presentation, please contact MS (email@example.com) or DHS (firstname.lastname@example.org).
Korean Association of Paleopathology and Osteoarchaeology (S Korea) and Japanese Association of Paleopathology will hold the Korea-Japan Paleopathology Forum on October, 2017.
Venue: Tokyo (University of Tokyo)
1. Eun Jin Woo, Hyunwoo Jung, Sunyoung Pak (Yonsei University College of Dentstry): Paleopathological study of a probablecase of treponematosis in a Joseon dynasty population.
2. Hiroshi Iijima, Shinji Harihara, Hitoshi Sumi, Kageyasu Takanashi, Yumi Ueda, Hata Junpei, Yohei Ishizawa, Lim Chun Ren, Ryo Matoba: DNA extaraction from ancient cremated bones using non-powdering sample pre-treatment method.
3. Jong Ha Hong, Chang Seok Oh, Min Seo, Dong Hoon Shin (Seoul National University): DNA Sequences of 18s rRNA and ITS2 genes obtained from ancient Trichuris trichiura eggs remained in Joseon Dynasty mummy coprolites.
4. Manabu Uetsuki: Horses in medieval Japan: paleopathological evidence of various usage.
5. Chang Seok Oh, Jong Ha Hong, Hye…
Session Title: Physical Anthropology
Day 2, Oct. 29, 2018 Organizers: Dong Hoon Shin (Seoul National University, South Korea) Myeung Ju Kim (Dankook University, South Korea)
13:30-13:55 mtDNA haplogroup in ancient human DNA samples by Kyung Yong Kim (Chung-Ang Uversity, Korea)
13:55-14:15 Caries, antemortem tooth loss and tooth wear observed in indigenous peoples and Russian settlers of 16th to 19th century West Siberia by Hye Jin Lee (Ministry of National Defense Agency KIA Recovery & Identification, Korea)
14:15-14:30 Parasite aDNA of Korean mummies by Jong Ha Hong (Seoul National University, Korea)
14:30-14:45 Famine and Stress Markers, with a Focus on Enamel Hypoplasia by Shiori Fujisawa (Aomori Chuo Gakuin University, Japan)
14:45-15:10 Arctic mummies in Salekhard by Dong Hoon Shin (Seoul National University, Korea)
Institute of Forensic Science, Seoul National University College of Medicine and Korean Academy of Paleopathology and Osteoarchaeology (KAPO) proudly invite you to “International Symposium: Current Trends in Paleoparasitology.”
Paleoparasitological research has advanced beyond the earliest stages of development, entering into a new stage to obtain information about our ancestors’ parasitic infections all over the world, using a variety of recently developed research techniques. This symposium will be an important opportunity to review the research achievements of paleoparasitology so far and to discuss current trends and future prospects in paleoparasitology with experts and specialists in the field.
For the details of the symposium, please click below: