[Presentation] 24th Annual Meeting of European Association of Archaeologists-.

JongHa Hong will present his (our) work in upcoming 24th Annual Meeting of European Association of Archaeologists on behalf of other members of my lab.

DNA Sequences of CO1 and ITS1 genes obtained from ancient Clonorchis sinensis eggs remained in Joseon Dynasty mummies

Jong Ha Hong1, Chang Seok Oh1,2, Min Seo3,*, Dong Hoon Shin1,2,*

1Laboratory of Bioanthropology, Paleopathology and History of Diseases, Seoul National University College of Medicine, Seoul, South Korea; 2Institute of Forensic Science, Seoul National University College of Medicine, Seoul, South Korea; 3Department of Parasitology, Dankook University College of Medicine, Cheonan, South Korea; *Co-correspondences. 

The parasitological information obtained from archaeological specimens can propose speculation about the parasite-infection patterns that prevailed in ancient societies. In this study, we thus analyzed Clonorchis sinensis (C. sinensis) DNA extracted from the ancient feces or liver of 16th-18th century Korean mummies, especially on Cytochrome C Oxidase Subunit 1 (CO1) and internal transcribed spacer 1 (ITS1) genes. The mummies (n=5; Mungyeong, Dalsung, Cheongdo, Hadong1 and Andong) found in the graves of Joseon period (1392-1910 CE) were used in this study. This kind of graves was used mainly by the upper-class people of Joseon period. The samples are ancient feces obtained from mummy intestine (Dalsung, Hadong1 and Andong); or the liver (Mungyeong and Cheongdo). After C. sinensis genes were successfully amplified from two mummies (Mungyeong and Andong), consensus sequences were determined by the alignment of the sequences of cloned PCR products. The obtained CO1 and ITS1 sequences were well matched with those of already reported C. sinensis, but can be clearly differentiated from other species such as Opisthorchis viverrini and Pseudamphistomum truncatum, etc. By the phylogenetic analysis of the ITS1 gene, we tried to see the clustering according to regional differences of the C. sinensis examined. This study shows the genetic characteristics of ancient C. sinensis in Joseon period of Korea by comparison with those of modern C. sinensis sequences worldwide. To improve the knowledge about C. sinensis evolution in much detail, C. sinensis gene sequences must be obtained from the regions of much wider geo-historical scope in forthcoming studies. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (no. NRF-2016R1A2B4015669). For any queries about this presentation, please contact MS (bbbenji@naver.com) or DHS (cuteminjae@gmail.com).