Dong Hoon Shin MD, PhD, MS. Lab of Bioanthropology, Paleopathology and History of Diseases, Department of Anatomy/Institute of Forensic Science, Seoul National University College of Medicine, 103 Daehak-ro (Yongon-dong), Jongno-gu, Seoul 03080, South Korea. E-mail: firstname.lastname@example.org(DH Shin); TEL: +82-2-740-8203
[Presentation] 24th Annual Meeting of European Association of Archaeologists-.
DNA Sequences of CO1 and ITS1 genes obtained from ancient Clonorchis sinensis eggs remained in Joseon Dynasty mummies
Jong Ha Hong1, Chang Seok Oh1,2, Min Seo3,*, Dong Hoon Shin1,2,*
1Laboratory of Bioanthropology, Paleopathology and History of Diseases, Seoul National University College of Medicine, Seoul, South Korea; 2Institute of Forensic Science, Seoul National University College of Medicine, Seoul, South Korea; 3Department of Parasitology, Dankook University College of Medicine, Cheonan, South Korea
The parasitological information obtained from archaeological specimens can propose speculation about the parasite-infection patterns that prevailed in ancient societies. In this study, we thus analyzed Clonorchis sinensis (C. sinensis) DNA extracted from the ancient feces or liver of 16th-18th century Korean mummies, especially on Cytochrome C Oxidase Subunit 1 (CO1) and internal transcribed spacer 1 (ITS1) genes. The mummies (n=5; Mungyeong, Dalsung, Cheongdo, Hadong1 and Andong) found in the graves of Joseon period (1392-1910 CE) were used in this study. This kind of graves was used mainly by the upper-class people of Joseon period. The samples are ancient feces obtained from mummy intestine (Dalsung, Hadong1 and Andong); or the liver (Mungyeong and Cheongdo). After C. sinensis genes were successfully amplified from two mummies (Mungyeong and Andong), consensus sequences were determined by the alignment of the sequences of cloned PCR products. The obtained CO1 and ITS1 sequences were well matched with those of already reported C. sinensis, but can be clearly differentiated from other species such as Opisthorchis viverrini and Pseudamphistomum truncatum, etc. By the phylogenetic analysis of the ITS1 gene, we tried to see the clustering according to regional differences of the C. sinensis examined. This study shows the genetic characteristics of ancient C. sinensis in Joseon period of Korea by comparison with those of modern C. sinensis sequences worldwide. To improve the knowledge about C. sinensis evolution in much detail, C. sinensis gene sequences must be obtained from the regions of much wider geo-historical scope in forthcoming studies. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (no. NRF-2016R1A2B4015669). For any queries about this presentation, please contact MS (email@example.com) or DHS (firstname.lastname@example.org).
Date: May 23-24, 2019 Venue: Jeju National University Hospital, Jeju Island, South Korea Organizing Committee: Dong Hoon Shin (Seoul National University) Myeung Ju Kim (Dankook University) Yuryang Jang (MND Agency for KIA Recovery & Identification) Eun Jin Woo (Sejong University) Scientific Committee: Soong Deok Lee (Seoul National University) Sunyoung Pak (Seoul National University) Dong Hoon Shin (Seoul National University) Hisashi Fujita (Niigata College of Nursing) Shinji Harihara (The University of Tokyo) Toshiyuki Tsurumoto (Nagasaki University) ▶ May 23, 2019 15:30 ~ 17:00 Korea-Japan Paleopathology Forum 2019 This forum was held in conjunction with The Annual Meeting of Korean Association of Physical Anthropologists 2019.
I am invited to the Editorial Board of the Springer series entitled "Bioarchaeology and Social Theory" . This series started in 2014, and since that time, 17 volumes have come out to push the boundaries of bioarchaeological and forensic inquiry into new and productive arenas.