Date: Oct 19-21, 2016
Venue: Yangyang, South Korea
>>Abstracts of My Lab
(1) The genetic analysis on ancient Ascaris discovered from archaeological sites in South Korea.
Chang Seok Oh1, Jong Ha Hong1, Min Seo2, Dong Hoon Shin1
1Department of Anatomy, Seoul National University College of Medicine, Korea
2Department of Parasitology, Dankook University College of Medicine, Korea
In paleoparasitological study, the genetic research on ancient Ascaris found in archaeological samples is very useful for physical anthropologists to elucidate the ancient-to-modern secular changes in parasitic infection pattern. However, the data on ancient Ascaris obtained up to now were too insufficient to reconstruct comprehensive evolutionary history of Ascaris spp. In this regard, we tried to get in much information on the genetic traits of ancient Ascaris by molecular studies on the archaeological samples. We analyzed Ascaris ancient DNA with various multiple genetic markers, obtaining consensus sequences of each ancient Ascaris gene, and further performing phylogenetic analyses on them. Our study clearly showed that the genetic characteristics of ancient Ascaris spp. prevalent in ancient Korea was not uniform but was diverse to a certain degree. Genetic analyses on ancient Ascaris also represented that they could be helpful in molecular diagnosis of ancient Ascaris infection. This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIP) (NRF-2016R1A2B4015669).
Keywords: Paleoparasitology, Ascaris, ancient DNA, Phylogenetic analysis, Korea
(2) Mitochondrial DNA Analysis of Bos taurus bones collected from Cheonggyecheon Archaeological site of Joseon Period
Jong Ha Hong a, Chang Seok Oh a, Chi Wook Cho b, Young Moon Shin c, Tae-sup Cho d, Dong Hoon Shin a, *
a Bioanthropology and Paleopathology Lab, Department of Anatomy and Cell Biology, Institute of Forensic Science, Seoul National University College of Medicine, Seoul, South Korea
b Seoul Museum of History, South Korea
c Seoul City Wall Division, Seoul Metropolitan Government, South Korea
d Department of History, Yonsei University, Seoul, South Korea
Although genetic information of Bos taurus has been successfully elucidated by a number of studies, there were very few researches on the ancient DNA of the cattle, especially made upon the historical cases that were raised in South Korea. In this study, we tried to analyze the mitochondrial DNA D-loop and coding region sequences of 15th century cattle bone obtained from Cheonggyecheon ruins of Joseon period. The consensus sequence was determined by the alignment of individual clone sequences. Consensus mitotype of the Cheonggyecheon cattle was 16138C, 106C, 109C, 221+C, 587+C, 2536A, 9682C, 13310C (Haplogroup=T3a). Comparing the current result with the sequences available in GenBank, Cheonggyecheon cattle mtDNA was similar to modern Bos taurus raised in South Korea, Japan, China (of northern provinces) and a few even in Europe, America and Oceania. Phylogenetic analysis also revealed that Cheonggyecheon B. taurus was genetically different from the cattle raised in and around Southern Chinese provinces. Although more studies on aDNA are still needed for enriching our genetic knowledge about the history of genus Bos, this report can be an important stepping stone for our future studies on the cattle of historical societies in East Asia.
(3) The first report of the anthropological research project on Zeleny Yar mummies in Russian Federation
Sergey Mikhailovich Slepchenko1,2,3, Alexander Vasilyevich Gusev4, Evgenia Olegovna Svyatova5, Jong Ha Hong6, and Dong Hoon Shin6
(1) Institute of the Problems of the Northern Development, Tyumen; (2) Institute of Archaeology and Ethnography, Novosibirsk, (3) Tyumen Scientific Center, Siberian Branch of the Russian Academy of Sciences, Tyumen, Russia; (4) Archeology Department, SPI YaNAO, Arctic Research Center, Salekhard, Russia; (5) Institution of Culture of Sverdlovsk Region, Ekaterinburg, Russia; (6) Dept. of Anatomy and Cell Biology, Seoul National University College of Medicine, Seoul, South Korea
Archaeologists have excavated the Zelyeny Yar archaeological site in Yamalo-Nenets Autonomus Okrug for the past several years. While they investigated two medieval necropolises, dated 9th to 13th centuries, seventy-two burials with a number of 12th to 13th century aboriginal Siberian mummies have been discovered. Although these mummies are very important to understand the physical and pathological traits of the Siberian people in history, scientific studies were not performed sufficiently on them by for now. In this regard, we, the Russian and South Korean researchers agreed to make collaborative studies on the Siberian aboriginal mummies. In the forthcoming years, our research will be mainly focused on a full and detailed anthropological picture about the pre-modern Siberian people, using various techniques such as archaeological, anatomical, histological, paleoparasitological, ancient DNA, stable isotope analysis, and craniofacial reconstruction. In fact, this is the first report of our long-term studies on invaluable 12th to 13th century Siberian Zeleny Yar mummies. Correspondences for this project: Sergey Mikhailovich Slepchenko (email@example.com) of Russian Federation or Dong Hoon Shin (firstname.lastname@example.org) of South Korea.