I will introduce herewith paleo-parasitological studies performed in my lab.
In general, the parasite eggs or larvae included in ancient samples were studied in two different ways: microscopic and molecular methods. Of course, simple decision like the presence of A. lumbricoides or T. trichiura could be also done in my lab. However, we need a collaborator-paleoparasitologist with higher technical skills for examining the samples in much details (e.g. finding rare parasite eggs within the samples). We already introduce Dr. Seo M., our collaborator for paleoparasitological study, in different page.
When the samples were collected in archaeological fields, they were treated for microscopic examination as follows:
1. Re-hydrated in 0.5% trisodium phosphate solution that had been periodically shaken over the course of one week.
2. The re-hydrated samples, after filtration through multiply layered gauze, were precipitated for one additional day.
3. After the upper turbid layer was discarded, the precipitates were dissolved again
4. Precipitate in solution was dropped onto the slides. They were examined by light microscope (Olympus, Japan).
Paleo-parasitological Studies in Asian Countries
Li J, Liao X and Yang H. Molecular Characterization of a Parasitic Tapeworm (Ligula) Based on DNA Sequences from Formalin-Fixed Specimens. 2000. 38(9-10) 309-322.
Wei D, Yang W, Huang S, Lu Y, Su T, Ma J, Hu W and Xie N. Parasitological investigation on the ancient corpse of the Western Han Dynasty unearthed from tomb No. 168 on Phoenix Hill in Jiangling county. Journal of Huazhong University of Science and Technology. 1981. 1(2) 16-23.
Liu W-Q, Liu J, Zhang J-H, Long X-C, Lei J-H and Li Y-L. Comparison of ancient and modern Clonorchis sinensis based on ITS1 and ITS2 sequences. Acta Tropica. 2007. 101 (2) 91-94.
Sha Z-L, Zhu C-D, Murphy RW, La Salle J, Huang D-W. Mitochondrial phylogeography of a leafminer parasitoid, Diglyphus isaea (Hymenoptera: Eulophidae) in China. Biological Control 38 (2006) 380–389.
Matsui A, Kanehara M, Kanehara M. Palaeoparasitology in Japan--discovery of toilet features. Mem Inst Oswaldo Cruz. 2003;98 Suppl 1:127-36.